Dr. Petra Janning

Project Group Leader, Chemical Biology

Research Focus

The mass spectrometry group provides infrastructure and service for mass spectrometric analysis of organic compounds. Mass spectrometry at the institute is divided into two parts: low-resolution mass spectrometry, which is organized in an open access mode whenever possible and high-resolution mass spectrometry and proteomics, which are organized in service mode.

“Low-resolution mass spectrometry”

Analysis of small molecules using GC/EI-MS, HPLC/ESI-MS, HPLC/APCI-MS, MALDI-MS, direct injection
Structure determination of low molecular-weight compounds using HPLC/ESI-MSn
Analysis of intact proteins using HPLC/ESI-MS, MALDI-MS
Preparative separations of small molecules using prep-HPLC/MS

“Proteomics”

For proteomics issues nano-HPLC/ESI-MS/MS instruments are used and data evaluation is performed in a qualitative or relative quantitative manner using mainly „label-free“ or SILAC approaches.

Protein identification after enzymatic digest
Relative quantification of proteins in complex mixtures up to complete lysates
Identification of target proteins of bioactive small molecules e.g. after „pull-down“ experiments
Identification of protein-protein interactions e.g. after immunoprecipitations
Identification of posttranslational modifications
Hydrogen-deuterium exchange mass spectrometry
Thermal Proteom Profiling (TPP)

Behind the link you find a TPP macro published in the following paper:

Reckzeh ES, Brockmeyer A, Metz M, Waldmann H, Janning P (2018). Target Engagement of Small Molecules: Thermal Profiling Approaches on Different Levels. Methods Mol Biol
doi: 10.1007/978-1-4939-8891-4_4