Delivering high quality recombinant proteins for protein crystallography

The Dortmund Protein Facility (DPF) is a high-throughput protein engineering and production unit of the Max-Planck Institute of Molecular Physiology in Dortmund, Germany.
The DPF acts as an institute-wide service centre and was designed to support the needs of structural-, chemical- and cell biological projects as well as biochemical HT-screening campaigns.

The DPF aims to promote and facilitate the use of high-throughput methods of protein engineering, production and analysis. By using cutting-edge technologies, the DPF seeks to achieve high-throughput production and engineering of proteins through automating, parallelizing and miniaturizing all stages of the involved processes.

The DPF has adapted and refined technologies for high-throughput cloning and protein isolation developed at the “Oxford Protein Production Facility”, which we gratefully acknowledge for their continues support. As well as automating and optimizing current technologies for each stage of the production process, the DPF is also developing novel techniques to address remaining bottlenecks.

An overview of the process and key technologies is tabulated on the DPF homepage, with further information accessible by following the menu on the top of each page.