Stefan Raunser

Structural Biochemistry

Phone:+49 (231) 133-2300
       www.raunser-lab.de

Assistance

Meike Schulte
Phone: +49 (231) - 133 2301
Fax: +49 (231) - 133 2399

Pospich S, Kumpula EP, von der Ecken J, Vahokoski J, Kursula I, Raunser S. (2017). Near-atomic structure of jasplakinolide-stabilized malaria parasite F-actin reveals the structural basis of filament instability. Proc Natl Acad Sci U S A
doi: 10.1073/pnas.1707506114

Efremov RG, Gatsogiannis C, Raunser S (2017). Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM. Methods in Enzymology
doi: https://doi.org/10.1016/bs.mie.2017.05.007.

Moriya T, Saur M, Stabrin M, Merino F, Voicu H, Huang Z, Penczek PA, Raunser S, Gatsogiannis C (2017). High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE. J Vis Exp
doi: 10.3791/55448.

Orekhov P, Bothe A, Steinhoff HJ, Shaitan KV, Raunser S, Fotiadis D, Schlesinger R, Klare JP, Engelhard M (2017). Sensory Rhodopsin I and Sensory Rhodopsin II Form Trimers of Dimers in Complex with their Cognate Transducers. Photochem Photobiol 93(3):796-804.
doi: 10.1111/php.12763.

Mosalaganti S, Keller J, Altenfeld A, Winzker M, Rombaut P, Saur M, Petrovic A, Wehenkel A, Wohlgemuth S, Müller F, Maffini S, Bange T, Herzog F, Waldmann H, Raunser S & Musacchio A (2017). Structure of the RZZ complex and molecular basis of its interaction with Spindly. J Cell Bio 216(4):961-981
doi: 10.1083/jcb.201611060.

Purushothaman LK, Arlt H, Kuhlee A, Raunser S, Ungermann C (2017). Retromer-driven membrane tubulation separates endosomal recycling from Rab7/Ypt7-dependent fusion. Mol Biol Cell pii: mbc.E16-08-0582.
doi: doi: 10.1091/mbc.E16-08-0582.

Kiontke S, Langemeyer L, Kuhlee A, Schuback S, Raunser S, Ungermann C, Kümmel D (2017). Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1-Ccz1. Nat Commun 8:14034.
doi: 10.1038/ncomms14034.


2016

Merino F, Raunser S (2016). The mother of all actins? eLife. 5: e23354.
doi: 10.7554/eLife.23354.

Merino F, Raunser S (2016). Cryo-EM as a tool for structure-based drug development. Angew Chem Int Ed Engl
doi: 10.1002/anie.201608432

Gatsogiannis C, Merino F, Serdiuk T, Prumbaum D, Roderer D, Leidreiter F, Meusch D, Müller DJ, and Raunser S (2016). Membrane insertion of a Tc toxin in atomic detail. Nature Structural and Molecular Biology 23(10):884-890.
doi: 10.1038/nsmb.3281
freely available!

von der Ecken J, Heissler SM, Pathan-Chhatbar S, Manstein DJ & Raunser S (2016).Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution. Nature 534(7609):724-28.
doi: 10.1038/nature18295
freely available!

Friese A, Faesen AC, Huis in´t Veld PJ, Fischböck J, Prumbaum D, Petrovic A, Raunser S, Herzog F, Musacchio A (2016). Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin. Nature Communications 7, 11407:1 - 11407:12.
doi: 10.1038/ncomms11407
freely available!

Liu Y, Petrovic A, Rombaut P, Mosalaganti S, Keller J, Raunser S, Herzog F, Musacchio A (2016). Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster. Open Biology 6 (2), S. 1-11.
doi: 10.1038/nsmb.3281


2015





von der Ecken J, Müller M, Lehman W, Manstein DJ, Penczek PA, Raunser S (2015). Structure of the F-actin-tropomyosin complex. Nature 519(7541):114-7.
doi: 10.1038/nature14033
freely available!

Frisch H, Nie Y, Raunser S, Besenius P (2015). pH-Regulated Selectivity in Supramolecular Polymerizations: Switching between Co- and Homopolymers. Chemistry – A European Journal 21 (8), S. 3304-3309.
doi: 10.1002/chem.201406281

Gao M, Berghaus M, von der Ecken J, Raunser S, Winter R (2015). Condensation Agents Determine the Temperature-Pressure Stability of F-Actin Bundles. Angewandte Chemie International Edition 54 (38), S. 11088-11092.
doi: 10.1002/anie.201504247

Kuhlee A, Raunser S, Ungermann C (2015). Functional homologies in vesicle tethering. FEBS Letters 589 (19 Pt A), S. 2487-2497.
doi: 10.1016/j.febslet.2015.06.001

Lürick A, Kuhlee A, Bröcker C, Kümmel D, Raunser S, Ungermann C (2015). The HABC domain of the snare vam3 interacts with the hops tethering complex to facilitate vacuole fusion. The Journal of Biological Chemistry 290 (9), S. 5405-5413.
doi: 10.1074/jbc.M114.631465

Patasi C, Godočíková J, Michlíková S, Nie Y, Káčeriková R, Kválová K, Raunser S, Farkašovský M (2015). The role of Bni5 in the regulation of septin higher-order structure formation. Biological Chemistry 396 (12), S. 1325-1337.
doi: 10.1515/hsz-2015-0165

Poepsel S, Sprengel A, Sacca B, Kaschani F, Kaiser M, Gatsogiannis C, Raunser S, Clausen T, Ehrmann M (2015). Determinants of amyloid fibril degradation by the PDZ protease HTRA1. Nature Chemical Biology 11 (11), S. 862-869.
doi: 10.1038/nchembio.1931
freely available!

Raunser S, Gatsogiannis C (2015). Deciphering the Tubulin Code. Cell 161 (5), S. 960-961.
doi: 10.1016/j.cell.2015.05.004

Whitney JC, Quentin D, Sawai S, LeRoux M, Harding B, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Raunser S, Mougous JD (2015). An Interbacterial NAD(P)+ Glycohydrolase Toxin Requires Elongation Factor Tu for Delivery to Target Cells. Cell 163 (3), S. 607-619.
doi: 10.1016/j.cell.2015.09.027

Efremov R, Hofnagel O, Raunser S (2015). Architecture and Conformational Switch Mechanism of the Ryanodine Receptor. Nature 517(7532):39-43.
doi: 10.1038/nature13916
freely available!

Gatsogiannis C, Hofnagel O, Markl J, Raunser S (2015). Structure of Mega-Hemocyanin Reveals Protein Origami in Snails. Structure 23 (1), S. 93-103.
doi: 10.1016/j.str.2014.10.013


2014





Basilico F, Maffini S, Weir J, Prumbaum D, Rojas AM, Zimniak T, De Antoni A, Jeganathan S, Voss B, Van Gerwen S, Krnn V, Massimiliano L, Valencia A, Vetter IR, Herzog F, Raunser S, Pasqualato S, Musacchio A (2014). The pseudo GTPase CENP-M drives human kinetochore assembly. eLife 3, e02978:1 - e02978:28.
doi: 10.7554/eLife.02978

Behrmann H, Lürick A, Kuhlee A, Balderhaar HK, Bröcker C, Kümmel D, Engelbrecht-Vandré S, Gohlke U, Raunser S, Heinemann U, Ungermann C (2014). Structural identification of the VPS18 β-propeller reveals a critical role in the HOPS complex stability and function. The Journal of Biological Chemistry 289 (48), S. 33503-33512.
doi: 10.1074/jbc.M114.602714

Kapoor S, Berghaus M, Suladze S, Prumbaum D, Grobelny S, Degen P, Raunser S, Winter R (2014). Prebiotic Cell Membranes that Survive Extreme Environmental Pressure Conditions. Angewandte Chemie International Edition 53 (32), S. 8397-8401.
doi: 10.1002/anie.201404254

Meusch D, Gatsogiannis C, Efremov R, Lang A, Hofnagel O, Vetter I, Aktories K, Raunser S (2014). Mechanism of Tc toxin action revealed in molecular detail. Nature 508(7494):61-5.
doi: 10.1038/nature13015
freely available!

Petrovic A, Mosalaganti S, Keller J, Mattiuzzo M, Overlack K, Krenn V, De Antoni A, Wohlgemuth S, Cecatiello V, Pasqualato S, Raunser S, Musacchio A (2014). Modular Assembly of RWD Domains on the Mis12 Complex Underlies Outer Kinetochore Organization. Molecular Cell 53 (4), S. 591-605.
doi: 10.1016/j.molcel.2014.01.019

Rosin C, Erlkamp M, von der Ecken J, Raunser S, Winter R (2014). Exploring the stability limits of actin and its suprastructures. Biophysical Journal 107 (12), S. 2982-2992.
doi: 10.1016/j.bpj.2014.11.006

Thakur HC, Singh M, Nagel-Steger L, Kremer J, Prumbaum D, Fansa EK, Ezzahoini H, Nouri K, Gremer L, Abts A, Schmitt L, Raunser S, Ahmadian MR, Piekorz RP (2014). The Centrosomal Adaptor TACC3 and the Microtubule Polymerase chTOG Interact via Defined C-terminal Subdomains in an Aurora-A Kinase-independent Manner. The Journal of Biological Chemistry 289 (1), S. 74-88.
doi: 10.1074/jbc.M113.532333


2013





Erkelenz M, Bauer DM, Meyer R, Gatsogiannis C, Raunser S, Saccà B, Niemeyer CM (2013). A Facile Method for Preparation of Tailored Scaffolds for DNA-Origami. Small 10 (1), S. 73-77.
doi: 10.1002/smll.201300701

Gatsogiannis C, Lang A, Meusch D, Pfaumann V, Hofnagel O, Benz R, Aktories K, Raunser S (2013). A syringe-like injection mechanism in Photorhabdus luminescens toxins. Nature. 495(7442):520-23
doi: 10.1038/nature11987
freely available!

Lakshminarasimhan M, Curth U, Moniot S, Mosalaganti S, Raunser S, Steegborn C (2013). Molecular architecture of the human protein deacetylase Sirt1 and its regulation by AROS and resveratrol. Bioscience Reports 33 (3) pii:e00037, S. 395-407.
doi: 10.1042/BSR20120121

Lehman W, Orzechowski M, Li XE, Fischer S, Raunser S (2013). Gestalt-Binding of tropomyosin on actin during thin filament activation. Journal of Muscle Research and Cell Motility 34 (3-4), S. 155-163.
doi: 10.1007/s10974-013-9342-0

Sadian Y, Gatsogiannis C, Patasi C, Hofnagel O, Goody RS, Farkasovský M, Raunser S (2013). The role of Cdc42 and Gic1 in the regulation of septin filament formation and dissociation. eLife 2, e01085:1 - e01085:26.
doi: 10.7554/eLife.01085

Schönichen A, Mannherz HG, Behrmann E, Mazur AJ, Kühn S, Silván U, Schoenenberger C-A, Fackler OT, Raunser S, Dehmelt L, Geyer M (2013). FHOD1 is a combined actin filament capping and bundling factor that selectively associates with actin arcs and stress fibers. Journal of Cell Science 126 (Pt8), S. 1891-1901.
doi: 10.1242/jcs.126706

Sot B, Behrmann E, Raunser S, Wittinghofer A (2013). Ras GTPase activating (RasGAP) activity of the dual specificity GAP protein Rasal requires colocalization and C2 domain binding to lipid membranes. PNAS 110 (1), S. 111-116.
doi: 10.1073/pnas.1201658110

Taft MH, Behrmann E, Munske-Weidemann L-C, Thiel C, Raunser S, Manstein DJ (2013). Functional Characterization of Human Myosin-18A and Its Interaction with F-actin and GOLPH3. he Journal of Biological Chemistry 288 (42), S. 30029-30041.
doi: 10.1074/jbc.M113.497180

Thakur HC, Singh M, Nagel-Steger L, Prumbaum D, Fansa EK, Gremer L, Ezzahoini H, Abts A, Schmitt L, Raunser S, Ahmadian MR, Piekorz RP (2013). Role of centrosomal adaptor proteins of the TACC family in the regulation of microtubule dynamics during mitotic cell division. Biological Chemistry 394 (11), S. 1411-1423.
doi: 10.1515/hsz-2013-0184


2012





Behrmann E, Müller M, Penczek PA, Mannherz HG, Manstein DJ, Raunser S (2012). Structure of the Rigor Actin-Tropomyosin-Myosin Complex. Cell 150 (2), S. 327-338.
doi: 10.1016/j.cell.2012.05.037

Behrmann E, Tao G, Stokes DL, Egelman EH, Raunser S, Penczek PA (2012). Real-space processing of helical filaments in SPARX. Journal of Structural Biology 177 (2), S. 302-313.
doi: 10.1016/j.jsb.2011.12.020

Bröcker C, Kuhlee A, Gatsogiannis C, kleine Balderhaar HJ, Hönscher C, Engelbrecht-Vandré S, Ungermann C, Raunser S (2012). Molecular architecture of the multisubunit homotypic fusion and vacuole protein sorting (HOPS) tethering complex. PNAS 109 (6), S. 1991-1996.
doi: 10.1073/pnas.1117797109

Hernandez JM, Stein A, Behrmann E, Riedel D, Cypionka A, Farsi Z, Walla PJ, Raunser S, Jahn R (2012). Membrane Fusion Intermediates via Directional and Full Assembly of the SNARE Complex. Science 336 (6088), S. 1581-1584.
doi: 10.1126/science.1221976

Müller M, Mazur AJ, Behrmann E, Diensthuber RP, Radke MB, Qu Z, Littwitz C, Raunser S, Schoenenberger C-A, Manstein DJ, Mannherz HG (2012). Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy. Cellular and Molecular Life Sciences 69 (20), S. 3457-3479.
doi: 10.1007/s00018-012-1030-5


2011





Schneider R, Schumacher MC, Mueller H, Nand D, Klaukien V, Heise H, Riedel D, Wolf G, Behrmann E, Raunser S, Seidel R, Engelhard M, Baldus M (2011). Structural Characterization of Polyglutamine Fibrils by Solid-State NMR Spectroscopy. Journal of Molecular Biology 412 (1), S. 121-136.
doi: 10.1016/j.jmb.2011.06.045


Before 2011





Wang L, Yang JK, Kabaleeswaran V, Rice AJ, Cruz AC, Park AY, Yin Q, Damko E, Jang SB, Raunser S, Robinson CV, Siegel RM, Walz T, Wu H (2010). The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Nature Structural and Molecular Biology 17 (11), S. 1324-1329.
doi: 10.1038/nsmb.1920

Park CY, Hoover PJ, Mullins FM, Bachhawat P, Covington ED, Raunser S, Walz T, Garcia KC, Dolmetsch RE, Lewis RS (2009). STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1. Cell 136 (5), S. 876-890.
doi: 10.1016/j.cell.2009.02.014

Raunser S, Magnani R, Huang Z, Houtz RL, Trievel RC, Penczek PA, Walz T (2009). Rubisco in complex with Rubisco large subunit methyltransferase. PNAS 106 (9), S. 3160-3165.
doi: 10.1073/pnas.0810563106

Raunser S, Walz T (2009). Electron Crystallography as a Technique to Study the Structure on Membrane Proteins in a Lipidic Environment. Annual Review of Biophysics 38, S. 89-105.
doi: 10.1016/j.cell.2009.02.014

Raunser S, Mathai JC, Abeyrathne PD, Rice AJ, Zeidel ML, Walz T (2009). Oligomeric structure and functional characterization of the urea transporter from Actinobacillus pleuropneumoniae. Journal of Molecular Biology 387 (3), S. 619-627.
doi: 10.1016/j.jmb.2009.02.005

Bankovich AJ, Raunser S, Juo ZS, Walz T, Davis MM, Garcia KC (2007). Structural insight into pre-B cell receptor function. Science 316 (5822), S. 291-294.
doi: 10.1126/science.1139412

Hite RK, Raunser S, Walz T (2007). Revival of electron crystallography. Current Opinion in Structural Biology 17 (4), S. 389-395.
doi: 10.1016/j.sbi.2007.06.006

Park HH, Logette E, Raunser S, Cuenin S, Walz T, Tschopp J, Wu H (2007). Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex. Cell 128 (3), S. 533-546.
doi: 10.1016/j.cell.2007.01.019

Yildiz Ö, Kalthoff C, Raunser S, Kühlbrandt W (2007). Structure of GlnK1 with bound effectors indicates regulatory mechanism for ammonia uptake. EMBO Journal 26 (2), S. 589-599.
doi: 10.1038/sj.emboj.7601492

Hobe S, Trostmann I, Raunser S, Paulsen H (2006). Assembly of the major light-harvesting chlorophyll-a/b complex: Thermodynamics and kinetics of neoxanthin binding. The Journal of Biological Chemistry 281 (35), S. 25156-25166.
doi: 10.1074/jbc.M604828200

Kim Y-G, Raunser S, Munger C, Wagner J, Song Y-L, Cygler M, Walz T, Oh B-H, Sacher M (2006). The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering. Cell 127 (4), S. 817-830.
doi: 10.1016/j.cell.2006.09.029

Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ (2006). A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312 (5779), S. 1526-1530.
doi: 10.1126/science.1128393

Raunser S, Appel M, Ganea C, Geldmacher-Kaufer U, Fendler K, Kühlbrandt W (2006). Structure and function of prokaryotic glutamate transporters from Escherichia coli and Pyrococcus horikoshii. Biochemistry 45 (42), S. 12796-12805.
doi: 10.1021/bi061008

Raunser S, Haase W, Franke C, Eckert GP, Müller WE, Kühlbrandt W (2006). Heterologously Expressed GLT-1 Associates in approximately 200-nm Protein-Lipid Islands. Biophysical Journal 91 (10), S. 3718-3726.
doi: 10.1529/biophysj.106.086900

Vinothkumar KR, Raunser S, Jung H, Kühlbrandt W (2006).Oligomeric structure of the carnitine transporter CaiT from Escherichia coli. The Journal of Biological Chemistry 281(8), S. 4795-4801.
doi: 10.1074/jbc.M508993200

Raunser S, Haase W, Bostina M, Parcej DN, Kühlbrandt W (2005). High-yield expression, reconstitution and structure of the recombinant, fully functional glutamate transporter GLT-1 from Rattus norvegicus. Journal of Molecular Biology351(3), S. 598-613.
doi: 10.1016/j.jmb.2005.06.036

 
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