Software Tools
NeuriteQuant: An open source toolkit for high content screens of neuronal morphogenesis
NeuriteQuant is a freely available open-source tool that enables automated analysis of neuronal morphologies [1]. It enables both analysis of individual experiments, as well as large-scale high-content screens. The analysis pipeline is particularly optimized to quantify the effect of experimental manipulations on physiologically relevant neuronal cultures that display a high degree of overlap among neurites, such as those that are derived from primary neurons, stem cells or brain sections.
![Figure 1: Flow Diagram of the NeuriteQuant Content Pipeline [1].](/4130549/original-1630605883.jpg?t=eyJ3aWR0aCI6ODQ4LCJmaWxlX2V4dGVuc2lvbiI6ImpwZyIsIm9ial9pZCI6NDEzMDU0OX0%3D--08b136a5f86ffbd2604d28371a9b55427c04386e "Figure 1: Flow Diagram of the NeuriteQuant Content Pipeline [1].")
![Figure 1: Flow Diagram of the NeuriteQuant Content Pipeline [1].](/4130549/original-1630605883.jpg?t=eyJ3aWR0aCI6MjQ2LCJvYmpfaWQiOjQxMzA1NDl9--b3f67828867064379c726a284268273c81b0336c)
The open-source software is provided as an ImageJ plugin and available here:
http://ewit.ccb.tu-dortmund.de/groups/CB/bastiaens/dehmelt/NeuriteQuant/
Relevant Literature:
[1] Dehmelt L, Poplawski G, Hwang E, Halpain S (2011)
NeuriteQuant: an open source toolkit for high content screens of neuronal morphogenesis.
BMC Neurosci 12:100
https://doi.org/10.1186/1471-2202-12-100