Molecular Dynamics of Cell Fate Decisions
Phone: +49 (231) 133-2223
Fax: +49 (231) 133-2299
The goal of our research is to understand how cell communication regulates cell fate decisions and cell differentiation. We study this question both with a focus on single cells as well as in cell populations. At the single-cell level, we investigate how quantities of extracellular signals are encoded in the dynamic activation patterns of intracellular signaling pathways, and how these dynamic patterns lead to the transcriptional programs that underlie stable differentiation. At the population level, we want to understand how recursive signaling interactions in cell assemblies give rise to the self-organized emergence of cell populations with reproducible proportions of cells with discrete fates. Our model system to address these questions are mouse embryonic stem cells (mESCs). mESCs are cultured pluripotent cells that retain the potential to differentiate into all cell types of the adult organism in vivo and in vitro, and that have the ability to self-organize into simple embryo-like structures. We use state-of-the-art reporter systems and long-term live-imaging of mESCs to study the dynamics of cell-fate decisions at several levels of organization.
WWe are currently offering the possibility to perform a Masters thesis on the topic “Development of an embryonic organoid system”. In this project, we want to establish a three-dimensional culture system of embryonic stem cells to recapitulate central aspects of early mammalian development in vitro. Excellent and motivated students with an interest to work at the interface between stem cell biology and synthetic embryology are encouraged to get in touch with us.
Schröter C, Rué P, Mackenzie JP, Martinez Arias A (2015).
FGF/MAPK signaling sets the switching threshold of a bistable circuit controlling cell fate decisions in embryonic stem cells.
Freyer L, Schröter C, Saiz N, Schrode N, Nowotschin S, Martinez Arias A, et al (2015). A loss-of-function and H2B-Venus transcriptional reporter allele for Gata6 in mice. BMC Dev Biol 15:38.
Mulvey CM, Schröter C, Gatto L, Dikicioglu D, Fidaner IB, Christoforou A, et al (2015). Dynamic Proteomic Profiling of Extra-Embryonic Endoderm Differentiation in Mouse Embryonic Stem Cells. Stem Cells 33:2712–25.
Martinez Arias, A., Nichols, J. and Schröter, C (2013). A molecular basis for developmental plasticity in early mammalian embryos. Development 140, 3499–3510.