A conformational sensor based on genetic code expansion reveals an autocatalytic component in EGFR activation
Baumdick M, Gelléri M, Uttamapinant C, Beránek V, Chin JW, Bastiaens PIH (2018). Nat Commun
The Bastiaens lab and the Chin lab developed a Förster resonance energy transfer (FRET)-based conformational EGFR sensor using genetic code expansion. Applying the sensor the group found a catalytic active conformation in EGFR monomers induced by phosphorylation of Y845.
This conformational transition depends on EGFR kinase activity and auto-phosphorylation on its C-termninal tail and leads to autocatalytic amplification of EGFR phosphorylation at low EGF dose.