Ingrid Vetter
Projektgruppenleiterin, Mechanistische Zellbiologie
Strukturaufklärung von Proteinen und Proteinkomplexen
Publikationen
2024
Porfetye AT, Stege P, Rebollido-Rios R, Hoffmann D, Schrader T, Vetter IR (2024). How Do Molecular Tweezers Bind to Proteins? Molecules
Source
Polley S, Raisch T, Ghetti S, Körner M, Terbeck M, Gräter F, Raunser S, Aponte-Santamaria Camilo, Vetter IR, Musacchio A (2024). Structure of the human KMN complex and implications for regulation of its assembly. NSMB
Source
2023
Polley S, Müschenborn H, Terbeck M, De Antoni A, Vetter IR, Dogterom M, Musacchio A, Volkov VA, Huis In 't Veld PJ (2023). Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding. EMBO J.
Source
2022
d'Amico EA, Ud Din Ahmad M, Cmentowski V, Girbig M, Müller F, Wohlgemuth S, Brockmeyer A, Maffini S, Janning P, Vetter IR, Carter AP, Perrakis A, Musacchio A (2022). Conformational transitions of the Spindly adaptor underlie its interaction with Dynein and Dynactin. J Cell Biol.
Source
Pesenti ME, Raisch T, Conti D, Walstein K, Hoffmann I, Vogt D, Prumbaum D, Vetter IR, Raunser S, Musacchio A (2022). Structure of the human inner kinetochore CCAN complex and its significance for human centromere organization. Mol Cell
Source
Raisch T, Ciossani G, d'Amico E, Cmentowski V, Carmignani S, Maffini S, Merino F, Wohlgemuth S, Vetter IR, Raunser S, Musacchio A (2022). Structure of the RZZ complex and molecular basis of Spindly-driven corona assembly at human kinetochores. EMBO J
Source
2021
Walstein K, Petrovic A, Pan D, Hagemeier B, Vogt D, Vetter I & Musacchio A (2021).Assembly principles and stoichiometry of a complete human kinetochore module. Science Advances
Source
Piano V, Alex A, Stege P, Maffini S, Stoppiello GA, Huis In 't Veld PJ, Vetter IR, Musacchio A (2021). CDC20 assists its catalytic incorporation in the mitotic checkpoint complex. Science
Source
2018
Schubert E, Vetter IR, Prumbaum D, Penczek PA, Raunser S (2018).Membrane insertion of α-xenorhabdolysin in near-atomic detail. eLife
doi: 10.7554/eLife.38017.
Bongard J, Lorenz M, Vetter IR, Stege P, Porfetye AT, Schmitz AL, Kaschani F, Wolf A, Koch U, Nussbaumer P, Klebl B, Kaiser M, Ehrmann M (2018). Identification of Noncatalytic Lysine Residues from Allosteric Circuits via Covalent Probes. ACS Chem Biol
doi: 10.1021/acschembio.8b00101.
2017
Vetter IR (2017).Interface analysis of small GTP binding protein complexes suggests preferred membrane orientations. Biol Chem
doi: 10.1515/hsz-2016-0287.
Pentakota S, Zhou K, Smith C, Maffini S, Petrovic A, Morgan GP, Weir JR, Vetter IR, Musacchio A, Luger K (2017). Decoding the centromeric nucleosome through CENP-N. Elife
doi: 10.7554/eLife.33442.
2016
Ursu A, Illich DJ, Takemoto Y, Porfetye AT, Zhang M, Brockmeyer A, Janning P, Watanabe N, Osada H, Vetter IR, Ziegler S, Schöler HR, Waldmann H (2016). Epiblastin A Induces Reprogramming of Epiblast Stem Cells Into Embryonic Stem Cells by Inhibition of Casein Kinase 1. Cell Chem Biol 23(4):494-507.
doi: 10.1016/j.chembiol.2016.02.015.
Huebinger J, Han HM, Hofnagel O, Vetter IR, Bastiaens PI, Grabenbauer M (2016).Direct Measurement of Water States in Cryopreserved Cells Reveals Tolerance toward Ice Crystallization. Biophys J 110(4):840-9.
doi: 10.1016/j.bpj.2015.09.029
2015
Rudack T, Jenrich S, Brucker S, Vetter IR, Gerwert K, Kötting C (2015). Catalysis of GTP hydrolysis by small GTPases at atomic detail by integration of X-ray crystallography, experimental, and theoretical IR spectroscopy. J Biol Chem290(40):24079-90.
doi: 10.1074/jbc.M115.648071.
Altenfeld A, Wohlgemuth S, Wehenkel A, Vetter IR, Musacchio A (2015). Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod-Zwilch-ZW10 (RZZ) complex. Acta Crystallogr F Struct Biol Commun 71(Pt 4):438-42.
doi: 10.1107/S2053230X15004343.
Meekrathok P, Bürger M, Porfetye AT, Vetter IR, Suginta W (2015).Expression, purification, crystallization and preliminary crystallographic analysis of a GH20 β-N-acetylglucosaminidase from the marine bacterium Vibrio harveyi. Acta Crystallogr F Struct Biol Commun71(Pt 4):427-33.
doi: 10.1107/S2053230X1500415X.
2014
Remans K, Bürger M, Vetter IR, Wittinghofer A (2014). C2 domains as protein-protein interaction modules in the ciliary transition zone. Cell Rep 8(1):1-9.
doi: 10.1016/j.celrep.2014.05.049.
Basilico F, Maffini S, Weir JR, Prumbaum D, Rojas AM, Zimniak T, De Antoni A, Jeganathan S, Voss B, van Gerwen S, Krenn V, Massimiliano L, Valencia A, Vetter IR, Herzog F, Raunser S, Pasqualato S, Musacchio A (2014). The pseudo GTPase CENP-M drives human kinetochore assembly. Elife 3:e02978.
doi: 10.7554/eLife.02978.
Meusch D, Gatsogiannis C, Efremov RG, Lang AE, Hofnagel O, Vetter IR, Aktories K, Raunser S (2014). Mechanism of Tc toxin action revealed in molecular detail. Nature508(7494):61-5.
doi: 10.1038/nature13015.
2013
Kemp LE, Rusch M, Adibekian A, Bullen HE, Graindorge A, Freymond C, Rottmann M, Braun-Breton C, Baumeister S, Porfetye AT, Vetter IR, Hedberg C, Soldati-Favre D (2013). Characterization of a serine hydrolase targeted by acyl-protein thioesterase inhibitors in Toxoplasma gondii. J Biol Chem288(38):27002-18.
doi: 10.1074/jbc.M113.460709.
Wätzlich D, Vetter I, Gotthardt K, Miertzschke M, Chen YX, Wittinghofer A, Ismail S (2013). The interplay between RPGR, PDEδ and Arl2/3 regulate the ciliary targeting of farnesylated cargo. EMBO Rep. 2013 May;14(5):465-72. doi: 10.1038/embor.2013.37.
doi: 10.1038/embor.2013.37.
Mihai Gazdag E, Streller A, Haneburger I, Hilbi H, Vetter IR, Goody RS, Itzen A (2013). Mechanism of Rab1b deactivation by the Legionella pneumophila GAP LepB. EMBO Rep 14(2):199-205.
doi: 10.1038/embor.2012.211.
Zimmermann TJ, Bürger M, Tashiro E, Kondoh Y, Martinez NE, Görmer K, Rosin-Steiner S, Shimizu T, Ozaki S, Mikoshiba K, Watanabe N, Hall D, Vetter IR, Osada H, Hedberg C, Waldmann H (2013). Boron-based inhibitors of acyl protein thioesterases 1 and 2. Chembiochem 14(1):115-22.
doi: 10.1002/cbic.201200571.
2012
Ismail SA, Chen YX, Miertzschke M, Vetter IR, Koerner C, Wittinghofer A (2012). Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119. EMBO J 31(20):4085-94.
doi: 10.1073/pnas.1203223109.
Görmer K, Bürger M, Kruijtzer JA, Vetter I, Vartak N, Brunsveld L, Bastiaens PI, Liskamp RM, Triola G, Waldmann H (2012). Chemical-biological exploration of the limits of the Ras de- and repalmitoylating machinery. Chembiochem 13(7):1017-23.
doi: 10.1002/cbic.201200078.
Rusch M, Zahov S, Vetter IR, Lehr M, Hedberg C (2012). Design, synthesis and evaluation of polar head group containing 2-keto-oxazole inhibitors of FAAH. Bioorg Med Chem 20(2):1100-12.
doi: 10.1073/pnas.1110553109.
Bürger M, Zimmermann TJ, Kondoh Y, Stege P, Watanabe N, Osada H, Waldmann H, Vetter IR (2012). Crystal structure of the predicted phospholipase LYPLAL1 reveals unexpected functional plasticity despite close relationship to acyl protein thioesterases. J Lipid Res 53(1):43-50.
doi: 10.1194/jlr.M019851.
Ismail SA, Chen YX, Miertzschke M, Vetter IR, Koerner C, Wittinghofer A (2012). Structural basis for Arl3-specific release of myristoylated ciliary cargo from UNC119. EMBO J 31(20):4085-94.
doi: 10.1073/pnas.1203223109.
Görmer K, Bürger M, Kruijtzer JA, Vetter I, Vartak N, Brunsveld L, Bastiaens PI, Liskamp RM, Triola G, Waldmann H (2012). Chemical-biological exploration of the limits of the Ras de- and repalmitoylating machinery. Chembiochem 13(7):1017-23.
doi: 10.1002/cbic.201200078.
Rusch M, Zahov S, Vetter IR, Lehr M, Hedberg C (2012). Design, synthesis and evaluation of polar head group containing 2-keto-oxazole inhibitors of FAAH. Bioorg Med Chem 20(2):1100-12.
doi: 10.1073/pnas.1110553109.
Bürger M, Zimmermann TJ, Kondoh Y, Stege P, Watanabe N, Osada H, Waldmann H, Vetter IR (2012). Crystal structure of the predicted phospholipase LYPLAL1 reveals unexpected functional plasticity despite close relationship to acyl protein thioesterases. J Lipid Res 53(1):43-50.
doi: 10.1194/jlr.M019851.
2011
Rusch M, Zimmermann TJ, Bürger M, Dekker FJ, Görmer K, Triola G, Brockmeyer A, Janning P, Böttcher T, Sieber SA, Vetter IR, Hedberg C, Waldmann H (2011). Identification of acyl protein thioesterases 1 and 2 as the cellular targets of the Ras-signaling modulators palmostatin B and M. Angew Chem Int Ed Engl 50(42):9838-42.
doi: 10.1002/anie.201102967.
Miertzschke M, Koerner C, Vetter IR, Keilberg D, Hot E, Leonardy S, Søgaard-Andersen L, Wittinghofer A (2011). Structural analysis of the Ras-like G protein MglA and its cognate GAP MglB and implications for bacterial polarity. EMBO J 30(20):4185-97.
doi: 10.1038/emboj.2011.291.
Zent E, Vetter I, Wittinghofer A (2011). Structural and biochemical properties of Sept7, a unique septin required for filament formation. Biol Chem 392(8-9):791-7.
doi: 10.1515/BC.2011.082.
Pantoom S, Vetter IR, Prinz H, Suginta W (2011). Potent family-18 chitinase inhibitors: x-ray structures, affinities, and binding mechanisms. J Biol Chem 286(27):24312-23
doi: 10.1074/jbc.M110.183376.
Wittinghofer A, Vetter IR (2011). Structure-function relationships of the G domain, a canonical switch motif. Annu Rev Biochem 80:943-71.
doi: 10.1146/annurev-biochem-062708-134043.
vor 2011
Filchtinski D, Sharabi O, Rüppel A, Vetter IR, Herrmann C, Shifman JM (2010). What makes Ras an efficient molecular switch: a computational, biophysical, and structural study of Ras-GDP interactions with mutants of Raf. J Mol Biol 399(3):422-35.
doi: 10.1016/j.jmb.2010.03.046.
Ismail SA, Vetter IR, Sot B, Wittinghofer A (2010). The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism. Cell 141(5):812-21. Erratum in: Cell. 2010 Dec 23;143(7):1190.
doi: 10.1016/j.cell.2010.03.051
Rosnizeck IC, Graf T, Spoerner M, Tränkle J, Filchtinski D, Herrmann C, Gremer L, Vetter IR, Wittinghofer A, König B, Kalbitzer HR (2010). Stabilizing a weak binding state for effectors in the human ras protein by cyclen complexes. Angew Chem Int Ed Engl 49(22):3830-3.
doi: 10.1002/anie.200907002.
Vetter IR (2009). Nup120: one more piece in the NPC puzzle. Structure17(8):1036-8.
doi: 10.1016/j.str.2009.07.005.
Schrader N, Koerner C, Koessmeier K, Bangert JA, Wittinghofer A, Stoll R, Vetter IR (2008). The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure16(7):1116-25.
doi: 10.1016/j.str.2008.03.014.
Schrader N, Stelter P, Flemming D, Kunze R, Hurt E, Vetter IR (2008). Structural basis of the nic96 subcomplex organization in the nuclear pore channel. Mol Cell 29(1):46-55.
doi: 10.1016/j.molcel.2007.10.022.
Sarić M, Zhao X, Körner C, Nowak C, Kuhlmann J, Vetter IR (2007). Structural and biochemical characterization of the Importin-beta.Ran.GTP.RanBD1 complex. FEBS Lett 581(7):1369-76.
doi: 10.1016/j.febslet.2007.02.067
Ziegler S, Röhrs S, Tickenbrock L, Möröy T, Klein-Hitpass L, Vetter IR, Müller O (2005). Novel target genes of the Wnt pathway and statistical insights into Wnt target promoter regulation. FEBS J 272(7):1600-15.
doi: 10.1111/j.1742-4658.2005.04581.x
Scrima A, Vetter IR, Armengod ME, Wittinghofer A (2005). The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J 24(1):23-33.
doi: 10.1038/sj.emboj.7600507
Daumke O, Weyand M, Chakrabarti PP, Vetter IR, Wittinghofer A (2004). The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature429(6988):197-201.
doi: 10.1038/nature02505
Shimada A, Nyitrai M, Vetter IR, Kühlmann D, Bugyi B, Narumiya S, Geeves MA, Wittinghofer A (2004). The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization. Mol Cell13(4):511-22.
http://dx.doi.org/10.1016/S1097-2765(04)00059-0
Dvorsky R, Blumenstein L, Vetter IR, Ahmadian MR (2004). Structural insights into the interaction of ROCKI with the switch regions of RhoA. J Biol Chem 279(8):7098-104.
doi: 10.1074/jbc.M311911200
Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR (2004). Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase. J Biol Chem 279(6):4743-9.
10.1074/jbc.M310281200
Seewald MJ, Kraemer A, Farkasovsky M, Körner C, Wittinghofer A, Vetter IR (2003). Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction? Mol Cell Biol 23(22):8124-36.
doi: 10.1128/MCB.23.22.8124-8136.2003 .
Häder T, Müller S, Aguilera M, Eulenberg KG, Steuernagel A, Ciossek T, Kühnlein RP, Lemaire L, Fritsch R, Dohrmann C, Vetter IR, Jäckle H, Doane WW, Brönner G (2003). Control of triglyceride storage by a WD40/TPR-domain protein. EMBO Rep 4(5):511-6.
doi: 10.1038/sj.embor.embor837.
Tickenbrock L, Cramer J, Vetter I, Muller O (2002). Structure of the amino-terminal nuclear export domain of the APC tumor suppressor protein. Ann N Y Acad Sci 973:109-11.
doi: 10.1111/j.1749-6632.2002.tb04616.x
Tickenbrock L, Cramer J, Vetter IR, Muller O (2002). The coiled coil region (amino acids 129-250) of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance region 1 (Crm-1). J Biol Chem 277(35):32332-8.
doi: 10.1074/jbc.M203990200.
Breinbauer R, Vetter IR, Waldmann H (2002). From protein domains to drug candidates-natural products as guiding principles in the design and synthesis of compound libraries. Angew Chem Int Ed Engl 41(16):2879-90. Review.
doi: 10.1007/978-3-662-05314-0_11.
Fiegen D, Blumenstein L, Stege P, Vetter IR, Ahmadian MR (2002). Crystal structure of Rnd3/RhoE: functional implications. FEBS Lett 525(1-3):100-4.
doi: 10.1016/S0014-5793(02)03094-6.
Seewald MJ, Körner C, Wittinghofer A, Vetter IR (2002). RanGAP mediates GTP hydrolysis without an arginine finger. Nature 415(6872):662-6.
doi: 10.1038/415662a .
Filchtinski D, Sharabi O, Rüppel A, Vetter IR, Herrmann C, Shifman JM (2010). What makes Ras an efficient molecular switch: a computational, biophysical, and structural study of Ras-GDP interactions with mutants of Raf. J Mol Biol 399(3):422-35.
doi: 10.1016/j.jmb.2010.03.046.
Ismail SA, Vetter IR, Sot B, Wittinghofer A (2010). The structure of an Arf-ArfGAP complex reveals a Ca2+ regulatory mechanism. Cell 141(5):812-21. Erratum in: Cell. 2010 Dec 23;143(7):1190.
doi: 10.1016/j.cell.2010.03.051
Rosnizeck IC, Graf T, Spoerner M, Tränkle J, Filchtinski D, Herrmann C, Gremer L, Vetter IR, Wittinghofer A, König B, Kalbitzer HR (2010). Stabilizing a weak binding state for effectors in the human ras protein by cyclen complexes. Angew Chem Int Ed Engl 49(22):3830-3.
doi: 10.1002/anie.200907002.
Vetter IR (2009). Nup120: one more piece in the NPC puzzle. Structure17(8):1036-8.
doi: 10.1016/j.str.2009.07.005.
Schrader N, Koerner C, Koessmeier K, Bangert JA, Wittinghofer A, Stoll R, Vetter IR (2008). The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure16(7):1116-25.
doi: 10.1016/j.str.2008.03.014.
Schrader N, Stelter P, Flemming D, Kunze R, Hurt E, Vetter IR (2008). Structural basis of the nic96 subcomplex organization in the nuclear pore channel. Mol Cell 29(1):46-55.
doi: 10.1016/j.molcel.2007.10.022.
Sarić M, Zhao X, Körner C, Nowak C, Kuhlmann J, Vetter IR (2007). Structural and biochemical characterization of the Importin-beta.Ran.GTP.RanBD1 complex. FEBS Lett 581(7):1369-76.
doi: 10.1016/j.febslet.2007.02.067
Ziegler S, Röhrs S, Tickenbrock L, Möröy T, Klein-Hitpass L, Vetter IR, Müller O (2005). Novel target genes of the Wnt pathway and statistical insights into Wnt target promoter regulation. FEBS J 272(7):1600-15.
doi: 10.1111/j.1742-4658.2005.04581.x
Scrima A, Vetter IR, Armengod ME, Wittinghofer A (2005). The structure of the TrmE GTP-binding protein and its implications for tRNA modification. EMBO J 24(1):23-33.
doi: 10.1038/sj.emboj.7600507
Daumke O, Weyand M, Chakrabarti PP, Vetter IR, Wittinghofer A (2004). The GTPase-activating protein Rap1GAP uses a catalytic asparagine. Nature429(6988):197-201.
doi: 10.1038/nature02505
Shimada A, Nyitrai M, Vetter IR, Kühlmann D, Bugyi B, Narumiya S, Geeves MA, Wittinghofer A (2004). The core FH2 domain of diaphanous-related formins is an elongated actin binding protein that inhibits polymerization. Mol Cell13(4):511-22.
http://dx.doi.org/10.1016/S1097-2765(04)00059-0
Dvorsky R, Blumenstein L, Vetter IR, Ahmadian MR (2004). Structural insights into the interaction of ROCKI with the switch regions of RhoA. J Biol Chem 279(8):7098-104.
doi: 10.1074/jbc.M311911200
Fiegen D, Haeusler LC, Blumenstein L, Herbrand U, Dvorsky R, Vetter IR, Ahmadian MR (2004). Alternative splicing of Rac1 generates Rac1b, a self-activating GTPase. J Biol Chem 279(6):4743-9.
10.1074/jbc.M310281200
Seewald MJ, Kraemer A, Farkasovsky M, Körner C, Wittinghofer A, Vetter IR (2003). Biochemical characterization of the Ran-RanBP1-RanGAP system: are RanBP proteins and the acidic tail of RanGAP required for the Ran-RanGAP GTPase reaction? Mol Cell Biol 23(22):8124-36.
doi: 10.1128/MCB.23.22.8124-8136.2003 .
Häder T, Müller S, Aguilera M, Eulenberg KG, Steuernagel A, Ciossek T, Kühnlein RP, Lemaire L, Fritsch R, Dohrmann C, Vetter IR, Jäckle H, Doane WW, Brönner G (2003). Control of triglyceride storage by a WD40/TPR-domain protein. EMBO Rep 4(5):511-6.
doi: 10.1038/sj.embor.embor837.
Tickenbrock L, Cramer J, Vetter I, Muller O (2002). Structure of the amino-terminal nuclear export domain of the APC tumor suppressor protein. Ann N Y Acad Sci 973:109-11.
doi: 10.1111/j.1749-6632.2002.tb04616.x
Tickenbrock L, Cramer J, Vetter IR, Muller O (2002). The coiled coil region (amino acids 129-250) of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance region 1 (Crm-1). J Biol Chem 277(35):32332-8.
doi: 10.1074/jbc.M203990200.
Breinbauer R, Vetter IR, Waldmann H (2002). From protein domains to drug candidates-natural products as guiding principles in the design and synthesis of compound libraries. Angew Chem Int Ed Engl 41(16):2879-90. Review.
doi: 10.1007/978-3-662-05314-0_11.
Fiegen D, Blumenstein L, Stege P, Vetter IR, Ahmadian MR (2002). Crystal structure of Rnd3/RhoE: functional implications. FEBS Lett 525(1-3):100-4.
doi: 10.1016/S0014-5793(02)03094-6.
Seewald MJ, Körner C, Wittinghofer A, Vetter IR (2002). RanGAP mediates GTP hydrolysis without an arginine finger. Nature 415(6872):662-6.
doi: 10.1038/415662a .