Molecular determinants of the Ska-Ndc80 interaction and their influence on microtubule tracking and force-coupling
Huis In 't Veld PJ, Volkov VA, Stender ID, Musacchio A, Dogterom M (2019). Elife
The groups of Andrea Musacchio and Marileen Dogterom from the Delft University of Technology revealed that the interaction of the two microtubule binding complexes Ska and Ndc80 is phosphorylation-dependent and does not require microtubules, applied force, or several previously identified functional determinants including the Ndc80-loop and the Ndc80-tail. Both, the Ndc80-tail and the Ndc80-bound Ska stabilize microtubule ends in a stalled conformation. Its duration predicts whether a microtubule is stabilized and rescued by the kinetochore.