Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation
Raisch T, Chang CT, Levdansky Y, Muthukumar S, Raunser S, Valkov E (2019). Nature Communications
doi: 10.1038/s41467-019-11094-z.
Tobias Raisch et al. reconstitute a complete, recombinant human CCR4-NOT complex, a conserved multiprotein complex which regulates eukaryotic gene expression via shortening of poly(A) tails of messenger RNA or by deadenylation of specific mRNA regions.
Using purified component variants the groups could unveil an additional layer of regulatory complexity to the human deadenylation machinery, which may prime it either for general or target-specific degradation.
A proposed mechanistic model for the switch from bulk to targeted deadenylation.