Dr. Katarzyna Kliza
Research Group Leader
Decoding Post-Translational Modification Signalling Networks
Overview
Successful cellular adaptation to continuously changing environmental conditions relies on intricate regulatory mechanisms. Post-translational modifications (PTMs) represent a key strategy to ensure a dynamic, specific and accurate cellular response to external and internal stimuli. These chemical modifications regulate all aspects of a protein’s life, such as folding, trafficking, interactions, and (enzymatic) activity. Hitherto, hundreds of different PTMs have been discovered and their associations with diverse human maladies are well-documented. Nevertheless, due to their complexity and intricate crosstalk, our understanding of the features and molecular and (patho)physiological roles of PTMs is far from being complete. Our group focuses on elucidating the fundamental mechanisms and the physiological relevance of PTM signalling networks, focusing on ADP-ribosylation. Towards this aim, we employ a multifaceted and interdisciplinary approach, including proteomics, biochemistry, molecular and cell biology techniques.
Publications
2023
Neikes, H. K., Kliza, K.W.‡, Gräwe, C.‡, Wester, R.A.‡, Jansen, P.W.T.C., Lamers, L.A., Baltissen, M.P., van Heeringen, S.J., Logie, C., Teichmann, S.A., Lindeboom, R.G.H.# and Vermeulen, M.# (2023) Quantification of absolute transcription-factor binding affinities in the native chromatin context using BANC-seq. In press
2021
Kliza, K.*#, Liu, Q.*, Roosenboom, L.R.M., Filippov, D.V.# and Vermeulen, M.# (2021) Reading ADP-ribosylation signalling using chemical biology and interaction proteomics. Mol Cell
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Kliza, K.*#, Song, W.*, Pinzuti, I., Schaubeck, S., Kunzelmann, S., Kuntin, D., Fornili, A., Pandini, A., Hofmann, K., Garnett, J.A., Stieglitz, B.#, and Husnjak, K.# (2021) N4BP1 is dimerization-dependent linear ubiquitin reader regulating TNFR1 signalling through linear ubiquitin binding and Caspase-8-mediated processing. bioRxiv
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2020
Kliza, K.# and Husnjak, K.# (2020) Resolving the complexity of ubiquitin networks. Front Mol Biosci
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2017
Kliza, K., Taumer, C., Pinzuti, I., Franz-Wachtel, M., Kunzelmann, S., Stieglitz, B., Macek, B., and Husnjak, K. (2017) Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry. Nat Methods
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2015
Leznicki, P.*, Korac-Prlic, J.*, Kliza, K., Husnjak, K., Nyathi, Y., Dikic, I., and High, S. (2015) Binding of SGTA to Rpn13 selectively modulates protein quality control. J Cell Sci
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* Co-first authors
# Co-corresponding authors
‡ Equally contributed authors