Dr. Katarzyna Kliza

Research Group Leader

Decoding Post-Translational Modification Signalling Networks

Overview

Successful cellular adaptation to continuously changing environmental conditions relies on intricate regulatory mechanisms. Post-translational modifications (PTMs) represent a key strategy to ensure a dynamic, specific and accurate cellular response to external and internal stimuli. These chemical modifications regulate all aspects of a protein’s life, such as folding, trafficking, interactions, and (enzymatic) activity. Hitherto, hundreds of different PTMs have been discovered and their associations with diverse human maladies are well-documented. Nevertheless, due to their complexity and intricate crosstalk, our understanding of the features and molecular and (patho)physiological roles of PTMs is far from being complete. Our group focuses on elucidating the fundamental mechanisms and the physiological relevance of PTM signalling networks, focusing on ADP-ribosylation. Towards this aim, we employ a multifaceted and interdisciplinary approach, including proteomics, biochemistry, molecular and cell biology techniques.

Publications

ORCID List

2024

Llerena Schiffmacher, DA*, Lee, SH*, Kliza, KW, Theil, AF, Akita, M, Helfricht, A, Bezstarosti, K, Gonzalo-Hansen, C, van Attikum, H, Verlaan-de Vries, M, Vertegaal, ACO, Hoeijmakers, JHJ, Marteijn, JA, Lans, H, Demmers, JAA, Vermeulen, M, Sixma, TK, Ogi, T, Vermeulen, W^#, and Pines, A^# (2024) The small CRL4^CSA ubiquitin ligase component DDA1 regulates transcription-coupled repair dynamics. Nat Commun
Source

Kliza, K.W.*^,^#, Song, W.*, Pinzuti, I., Schaubeck, S., Kunzelmann, S., Kuntin, D., Fornili, A., Pandini, A., Hofmann, K., Garnett, J.A., Stieglitz, B.^#, and Husnjak, K.^#(2024) N4BP1 functions as a dimerization-dependent linear ubiquitin reader which regulates TNF signalling. Cell Death Discov.
Source

2023

Llerena Schiffmacher DA*, Lee SH*, Kliza KW, Theil AF, Akita M, Helfricht A, Bezstarosti K, Gonzalo-Hansen, C, van Attikum H, Verlaan-de Vries M, Vertegaal A, Hoeijmakers J, Marteijn J, Lans H, Demmers JAA, Vermeulen, M, Sixma, T, Ogi T, Vermeulen W^#, and Pines A.^#(2023). DDA1, a novel factor in transcription-coupled repair, modulates CRL4^CSA dynamics at DNA damage-stalled RNA polymerase II. In revision.

Llerena Schiffmacher DA, Kliza KW, Theil AF, Kremers GJ, Demmers JAA, Ogi T, Vermeulen M., Vermeulen W^# and Pines A^# (2023). Live cell transcription-coupled nucleotide excision repair dynamics revisited. DNA Repair (Amst)
Source

Neikes HK, Kliza KW^‡, Gräwe C^‡, Wester RA^‡, Jansen PWTC, Lamers, LA, Baltissen MP, van Heeringen SJ, Logie C, Teichmann SA, Lindeboom RGH^# and Vermeulen M^# (2023) Quantification of absolute transcription-factor binding affinities in the native chromatin context using BANC-seq. Nat Biotechnol
Source

2021

Kliza, K.*^#, Liu, Q.*, Roosenboom, L.R.M., Filippov, D.V.^# and Vermeulen, M.^# (2021) Reading ADP-ribosylation signalling using chemical biology and interaction proteomics. Mol Cell
Source

Kliza, K.*^#, Song, W.*, Pinzuti, I., Schaubeck, S., Kunzelmann, S., Kuntin, D., Fornili, A., Pandini, A., Hofmann, K., Garnett, J.A., Stieglitz, B.^#, and Husnjak, K.^# (2021) N4BP1 is dimerization-dependent linear ubiquitin reader regulating TNFR1 signalling through linear ubiquitin binding and Caspase-8-mediated processing. bioRxiv
Source

2020

Kliza, K.^# and Husnjak, K.^# (2020) Resolving the complexity of ubiquitin networks. Front Mol Biosci
Source

2017

Kliza, K., Taumer, C., Pinzuti, I., Franz-Wachtel, M., Kunzelmann, S., Stieglitz, B., Macek, B., and Husnjak, K. (2017) Internally tagged ubiquitin: a tool to identify linear polyubiquitin-modified proteins by mass spectrometry. Nat Methods
Source

2015

Leznicki, P.*, Korac-Prlic, J.*, Kliza, K., Husnjak, K., Nyathi, Y., Dikic, I., and High, S. (2015) Binding of SGTA to Rpn13 selectively modulates protein quality control. J Cell Sci
Source

^* Co-first authors
^# Co-corresponding authors
^‡Equally contributed authors